%0 Journal Article
%T Establishment of multiple shoot clumps from maize (Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets<br>Establishment of multiple shoot clumps from maize(Zea mays L.)and regeneration of herbicide-resistant transgenic plantlets
%A LI Guosheng
%A ZHANG Qingwei
%A ZHANG Juren
%A BI Yuping
%A SHAN Lei
%A <br>李国圣
%A 张卿伟
%A 张举仁
%A 毕玉平
%A 单雷
%J 中国科学C辑(英文版)
%D 2002
%I Springer
%X A kind of quick, efficient and season-free inducing embryoid and multiple shoot clumps system from shoot tip meristems that derived from elite inbreds of maize was established. The herbicide-resistant gene a/s (coding Acetolactate synthase) isolated from a mutant of Arabidopsis thaliana was transferred to tissue pieces of maize multiple shoot clumps by microprojectile bombardment. Herbicide-resistant tissue and regenerants were obtained through selections with herbicide chlorsulfuron. PCR analysis and Southern blot hybridization indicated that gene a/s has been transferred to some regenerants. The test of spraying chlorsulfuron displayed that the transgenic plantlets and R1 plants had favorable herbicide-resistant trait. We have established a new genotype-free system of maize which could rapidly and efficiently produce large quantities of transgenic plantlets.
%K maize
%K multiple shoot clumps
%K transgene
%K als
%K chlorsulfuron<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=6A2ED1D288C9F570E333396B62067B41&yid=C3ACC247184A22C1&vid=94E7F66E6C42FA23&iid=CA4FD0336C81A37A&sid=1371F55DA51B6E64&eid=2A3781E88AB1776F&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=16