%0 Journal Article
%T Transformation of Populus euphratica<br>胡杨转基因体系的建立
%A Yuanwei Fan
%A Aizhi Liu
%A Huafang Wang
%A <br>范源伟
%A 刘挨枝
%A 王华芳
%J 植物学报
%D 2009
%I 
%X The uniquearbor tree species Populus euphratica can grow well in arid desert. We established an Agrobacterium based transformation system for this species using a GUS gene as the reporter. Shoots of P. euphratica were regenerated from leaves placed on 1/2 MS supplemented with 1.0 mmol.L-1 6-BA and 5.0 mmol.L-1 NAA, leaves were infected for 15 min in a solution of A. tumefaciens with 0.4-0.6 OD600 and 100 mmol.L-1 AS, and then co-cultured for 2 days. A. tumefaciens was inhibited with 300 mg.L-1 Cef, and transgenic tissues and shoots were selected with 9 mg.L-1 G418.
%K <br>根癌农杆菌
%K GUS基因
%K 胡杨
%K 抗逆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=B764515ED6E9F747D2D3556E492F5602&yid=DE12191FBD62783C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=4D1A534FF6CD5D9A&eid=7F9B7E84827A650F&journal_id=1674-3466&journal_name=植物学报&referenced_num=1&reference_num=9