%0 Journal Article
%T Inhibition of metastasis to lung of a human nasopharyngeal carcinoma cell line CNE-2L2 transfected with pRc/CMV-antisense 6A8 cDNA in nude mice
%A ZHANG Lixin
%A GU Pei
%A LI Bo
%A ZHANG Shuzhen
%A LIU Yuqin
%A SHI Gengxian
%A GAO Jin
%A LI Guoyan
%A ZHU Liping
%A MA Fengrong
%A ZHAO Xuemei ZHAO Fangtao WANG Xun
%A <br>张立新
%A 刘玉琴
%A 马凤蓉
%A 顾蓓
%A 史耕先
%A 赵雪梅
%A 李波
%A 高进
%A 赵方萄
%A 张淑珍
%A 李国燕
%A 王讯
%A 朱立平
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X The growth of CNE-2L2 cell, a cloned line of human nasopharyngeal carcinoma with a high potentiality of metastasis to lung was inhibited to a certain extent after transfection with a recombinant antisense expression vector of a cDNA encoding a human a-mannosidase (pRc/CMV-antisense 6A8 cDNA) (the Genbank accession number of 6A8 cDNA is U37248) in comparison with that of the cell transfected with the Mock and of the wild cell. Two months after a subcutaneous inoculation of CNE-2L2 cell into the axilla of nude mice metastatic lesions in the lung were observed in 9/10 mice (90%) with grade III in 8 mice and grade II in one mouse in the wild cell group, in 6/8 mice (75%) with grade III in one mouse, grade II in 2 mice and grade I in 3 mice in the Mock-transfection group, in only 3/10 mice (30%) with all grade I in pRc/CMV-antisense 6A8 cDNA-transfection group.
%K antisense 6A8 cDNA
%K transfection
%K metastasis
%K nasopharyngeal carcinoma cell line
%K α
%K -mannosidase<br>antisense
%K 6A8
%K cDNA
%K transfection
%K metastasis
%K nasopharyngeal
%K carcinoma
%K cell
%K line
%K α-mannosidase.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=95A85D6FC74094396869B3CF6C0CAF27&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=0B39A22176CE99FB&sid=79D2EF35F60110C2&eid=1B64850025D0BBBE&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=3&reference_num=12