%0 Journal Article
%T A Rapid Micro-volume Method for Determining b-amylase Activity in Plant Seeds<br>一种微量、快速测定植物种子b- 淀粉酶活性的方法
%A Tengfang Ling
%A Jinshan Lin
%A Hui Liu
%A Bo Zhang
%A Jing Li
%A Tian Yu
%A Maobing Ye
%A Wenbiao Shen
%A <br>凌腾芳
%A 林锦山
%A 刘辉
%A 张博
%A 李静
%A 余阗
%A 叶茂炳
%A 沈文飚
%J 植物学报
%D 2006
%I 
%X In view of the disadvantages of the 3,5-dinitrosalicylic acid (DNS) method, we developed a rapid micro-volume procedure utilizing p-nitrophenyl-a-maltopentaoside (PNPG5) as a substrate for specific determination of b-amylase activity. The assay procedure was evaluated with seeds from 4 plant species, and the exact degree of interference by a-amylase was determined. This modified method could be applied to the selective measurement of b-amylase activity in different plant seeds.
%K <br>b-淀粉酶
%K 测活方法
%K 对硝基苯酚麦芽五糖
%K 植物种子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=230FDD80C997DDD5BAE7DFE575A27F22&yid=37904DC365DD7266&vid=EA389574707BDED3&iid=38B194292C032A66&sid=C1B34927D429E92F&eid=B8F8200D88DDC7D6&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=7