%0 Journal Article
%T Purification of an Antifreeze Protein from Ligustrum lucidum Leaves and Its Partial Characteristics<br>一种女贞叶抗冻蛋白的分离纯化
%A Shang Liu
%A Xiangru Liao
%A Jianguo Zhang
%A Huijuan Miao
%A <br>刘尚
%A 廖祥儒
%A 张建国
%A 苗会娟
%J 植物学报
%D 2007
%I 
%X According to the ice-binding characteristic, antifreeze proteins in the leaves of Ligustrum lucidum were isolated by adsorption to ice pieces at 0°C. Using Sephadex G-100 gel filtration and DEAE cellulose-52 anion exchange chromatography, we obtained 4 protein fractions, including a 36 kDa protein, identified as antifreeze proteins by differential scanning calorimetry. The THA value of the protein was approximately 0.678 °C when the protein concentration was 5 mg.mL-1. The maximal absorption peak was 975 nm after full-spectrum scanning from 200 to 1 000 nm. The content of hydrophilic amino acids was relatively higher than that of the other proteins.
%K <br>抗冻蛋白
%K 性质
%K 冰结合
%K 女贞
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=77513D848419ACC4C21A789BA1565121&yid=A732AF04DDA03BB3&vid=B91E8C6D6FE990DB&iid=E158A972A605785F&sid=42D7028D961473F8&eid=25467A5A28500A25&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=21