%0 Journal Article
%T Acquirement and characterization of a carotenoid mutant (GM309) of Rhodobacter sphaeroides 601
%A LIU Yuan
%A ZHANG Wei
%A WU Yongqiang
%A XU Chunhe
%A <br>LIU Yuan
%A ZHANG Wei
%A WU Yongqiang & XU Chunhe Shanghai Institute of Plant Physiology & Ecology
%A Shanghai institute for Biological Sciences
%A Chinese Academy of Sciences
%A Shanghai
%A China
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X A green mutant was obtained among the chemically induced mutants of Rhodo-bacter sphaeroides 601 (RS601) and named GM309. A blue shift of 20 nm of the carotenoid absorption spectrum was found in the light-harvesting complex II (LH2) of GM309. Different from LH2 of RS601, it was found that the carotenoids in GM309-LH2 changed to be neurosporene by mutation. Neurosporene lacks a conjugate double bond, compared with the spheroidene in RS601-LH2 which has ten conjugate double bonds. As shown by absorption and circular di-chroism spectroscopy, the overall structure of GM309-LH2 is little affected by this change. From fluorescence emission spectra, it is found that GM309-LH2 can transfer energy from carotenoids to Bchl-B850 without any change in efficiency. But the efficiency of energy transfer from B800 to B850 in GM309-LH2 is decreased to be 42% of that of the native. This work would provide a novel method to investigate the mechanism of excitation energy transfer in LH2.
%K light-harvesting complex
%K carotenoid
%K excitation energy transfer
%K fluorescence<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=6DE59262D4E47087E27F6636BBFFE1A8&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=CA4FD0336C81A37A&sid=286FB2D22CF8D013&eid=9FFCC7AF50CAEBF7&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15