%0 Journal Article
%T Establishment of Callus Regeneration System for Acer ginnala Maxim and Determination of Gallic Acid in Callus<br>茶条槭愈伤组织的再生体系建立及其没食子酸含量的测定
%A Haiyan Li
%A Jiyuan Song
%A Jie Dong
%A Yaguang Zhan
%A <br>李海艳
%A 宋继园
%A 董杰
%A 詹亚光
%J 植物学报
%D 2008
%I 
%X A reproducible system for callus induction of Acer ginnala Maxim is described. The system involves mature seeds sprouted on MS medium supplemented with 0.1 mg·L-1 6-BA, the stems of young seedling as explants, and calli easily induced on woodyplant medium (WPM) supplemented with 0.002-0.01 mg·L-1 TDZ and 0.1 mg·L-1 6-BA 3 weeks following induction. The frequency of callus induction can be as high as 98.0%. On WPM containing 0.01 mg·L-1 TDZ and 0.1 mg·L-1 6-BA, 42% of calli differentiated, and the mean number of buds per piece of callus was about 10. The buds developed roots on WPM medium with 0.3 mg·L-1 IBA and formed plantlets, 89% of which survived on transplantation to the greenhouse. Methods to extract and determine gallic acid from callus were established and showed 2.8% content of gallic acid in bottles of green callus subcultured twice.
%K <br>茶条槭
%K 愈伤组织
%K 没食子酸
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=3362A4201BDCF89AB114B2751C6614C3&yid=67289AFF6305E306&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=D2742EEE6F4DF8FE&eid=D5C73DEF4CF8FAF3&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=20