%0 Journal Article
%T Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin
%A ZHANG Zhou
%A TANG Yuehua
%A YAO Shiyin
%A ZHU Shangquan
%A FENG Youmin
%A <br>张舟
%A 唐月华
%A 姚矢音
%A 朱尚权
%A 冯佑民
%J 中国科学C辑(英文版)
%D 2003
%I Springer
%X Blood glucose lowering assay proved that B16Ala]insulin and B26Ala]insulin exhibit potency of acute blood glucose lowering in normal pigs, which demonstrates that they are fast- acting insulin. Single-chain precursor of B16Ala]insulin and B26Ala]insulin is B16Ala]PIP and B26Ala]PIP, respectively, which are suitable for gene expression. Secretory expression level of the precursors in methylotrophic yeast Pichia pastoris was quite high, 650 mg/L and 130 mg/L, respectively. In vivo biological assay showed that the two fast-acting insulins have full or nearly full biological activity. So both B16Ala]insulin and B26Ala]insulin can be well developed as fast-acting insulin for clinic use.
%K insulin
%K monomeric insulin
%K fast-acting insulin
%K Pichia pastoris<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=73062C22017075B0BEBF1EB2FFCB8C49&yid=D43C4A19B2EE3C0A&vid=D997634CFE9B6321&iid=94C357A881DFC066&sid=FE6B7E9BDCCDBAA6&eid=03436AC72A659ACA&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=18