%0 Journal Article
%T The screening and identification of endogenous retrovirus free CEMPs
%A LU Quanzhi
%A HAN Hongbing
%A LIAN Zhengxing
%A LI Ning
%A ZHANG Qingcai ZHAO Zili PEI Dezhi ZHANG Xiaolan WU Changxin
%A <br>LU Quanzhi
%A HAN Hongbing
%A LIAN Zhengxing
%A LI Ning
%A ZHANG Qingcai
%A ZHAO Zili
%A PEI Dezhi
%A ZHANG Xiaolan & WU Changxin . College of Animal Science and Technology
%A China Agricultural University
%A Beijing
%A China
%A . State Key Laboratory of Agrobiotechnology
%A Beijing
%A China
%A . Laboratory of Animal Genetics and Breeding
%A Ministry of Agriculture
%A Beijing
%A China
%A . Sangzi Hu Pig Breeding Farm
%A Hubei
%A China Correspondence should be addressed to Lian Zhengxing
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X The provirus DNA sequence of porcine endogenous retrovirus (PERV) distributed in the pig genome is the major obstacle that restricts the swine as the organ donors in xenotransplantation, and the copy number of PERV varies greatly among different breeds and individuals. In the experiment, 67 healthy, female Chinese Experimental Mini-Pigs (CEMPs) aged at 3-6 months were selected from the Animal Husbandry Station of China Agricultural University, the copy number of PERV and types of envelope protein gene (env) were then investigated by means of PCR analysis and Southern blotting. It is showed that the distribution of types of envelope protein gene in Landrace and CEMPs makes little difference, but the proportion of individuals carrying two types of envelope protein gene (env-A and env-B, which is denoted as env-AB) is much larger than those which carry only one type of envelope protein gene (env-A or env-B). Meanwhile, two endogenous retrovirus free pigs were found for the first time during our research, and the copy number of others is relatively low, which is about 10 to 20. All the results illuminate the genetic diversity of indigenous pig breeds in China and the potential of CEMPs to serve as organ donors in xenotransplantation.
%K swine
%K CEMPs
%K porcine endogenous retrovirus (PERV)
%K PCR
%K Southern blot<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=E6B2E1F4C207B44119C63DE61ED6B102&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=B31275AF3241DB2D&sid=12AD09BCF4A6E651&eid=CDC418F38C4BFD60&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=8