%0 Journal Article
%T Effect of DNA methylation on protein-DNA interaction of HL-60 cells
%A HE Zhongxiao
%A BAI Jianshi
%A ZHANG Yu
%A <br>何忠效
%A 白坚石
%A 张昱
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as inducer in the presence of optimum concentration of 10 pmol/L. The methylation level of genome DNA determined by HPLC is increased during cell differentiation. When restriction endonucleaseHae III,Sma I,Sal I,XhoI andHind III which are sensitive to 5-methyicytoside were used to cleave the genome DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.
%K S-Adenosyl-L-methionine (SAM)
%K HL-60 cell
%K DNA methylation
%K protein-DNA interaction<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=D4B2AA98E0E77BB8124A4A3E24F3DCF5&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=94C357A881DFC066&sid=3054A11AD1D7E34A&eid=42D7028D961473F8&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=7