%0 Journal Article
%T The interaction between ADAM22 and 14-3-3β
%A ZHU Pengcheng
%A SANG Yingying
%A XU Rener
%A ZHAO Jing
%A LI Changben
%A ZHAO Shouyuan
%A <br>
%J 中国科学C辑(英文版)
%D 2002
%I Springer
%X ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities.Adam22 is highly expressed in human brain. Theadam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved byin vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.
%K ADAM22
%K 14-3-3β
%K yeast two-hybrid assay
%K immunoprecipitation
%K cell adhesion<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=8CB1D624A649A3B717FC851E1907A73B&yid=C3ACC247184A22C1&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=52B9DFFFCC2EB041&eid=90773C2285A2F0BB&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=18