%0 Journal Article
%T Isolation of ScFv antibodies of rP27Kip1 from phage display libraries constructed from immunized and non-immunized repertoires<br>
%A CAO Yueqiong QIAO Shouyi YUAN YouzhongHUANG Jiansheng ZHAO Shouyuan
%A <br>
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X Through mRNA extract, RT and a series of PCR, phage antibody libraries were constructed from rP27kip 1-immunized and non-immunized mice. After only one round of selection with rP27kip 1, clones from each library were chosen randomly and digested byTag I and Hinf I. 11 of 64 clones from the immunized animal had consistent restriction pattern, while none of the 64 clones from the non-immunized animal had, except that one had the same fragments pattern as that of the 11 clones. The 12 fragments were expressed inE. coli BL21(DE3)/pET-28b(+) system. ELISA showed that some of the fragments could bind to rP27kip 1 specifically. AU these results implied that specific antibody can be obtained by genetic engineering without hybridoma or many rounds of growth and panning selection.
%K rP27
%K kip 1
%K phage antibody Libraries
%K ScFv
%K expression inE<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=3CC65544B0C4767372F7DFEC5057753E&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=09D368C679EC819B&eid=9C82B18080268586&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=9