%0 Journal Article
%T Molecular replacement study on form-B monoclinic crystal of insulin
%A DING Jinhui
%A WAN Zhuli
%A CHANG Wenrui
%A LIANG Dongcai
%A <br>定瑾晖
%A 万柱礼
%A 常文瑞
%A 梁栋材
%J 中国科学C辑(英文版)
%D 1996
%I Springer
%X The form-B monodinic insulin crystal was obtained from the sodium citrate buffer with 1% zinc chloride, keeping phenolic content between 0.76% and 1.25%. Its space group is P21, cell constants are: a = 4.924nm, b=6.094nm, c=4.818nm, β=95.8°. There are 6 insulin molecules which form a hexamer. The initial phase was obtained by using rotation function program of X-PLOR program package and molecular packing program of our laboratory. The molecular model was chosen from 4 zinc bovine insulin hexamer. After the preliminary refinement by using the rnacromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemically restrained least-squared refinement on the difference Fourier maps. The finial R-factor is 214% at 0.3nm resolution, the r.m.s. deviations from standard bond length and bond angle are 0.0022nm and 4.7°, respectively.
%K form-B monoclinic insulin crystal
%K molecular replacement method
%K molecular packing method<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=7A05D678E04B95E780543389D967AA5D&yid=8A15F8B0AA0E5323&vid=7C3A4C1EE6A45749&iid=0B39A22176CE99FB&sid=3986B25773CB6C30&eid=D59111839E7C8BDF&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=0