%0 Journal Article
%T Analyzing the H19- and T65-epitopes in 38 kd phosphorylated protein of Marek’s disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes<br>Analyzing the H19- and T65-epitopes in 38 kd phosphory-lated protein of Marek’s disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes
%A CUI Zhizhong
%A ZHANG Zhi
%A QIN Aijian
%A Lee Lucy F
%A <br>Lee
%A Lucy
%A F
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X DNA sequencing analysis in 38 kd phosphorylated protein (pp38) ORF of Marek's disease viruses (MDV) indicated that all tested 10 virulent strains with different pathotypes had 'A' at base #320 and glutamine at aa#107 while reacted with monoclonal antibody (Mab) H19 in indirect fluorescence antibody test (IFA). However, vaccine strain CVI988 had 'G' at base#320 and arginine at aa#107 instead, when it was negative in IFA with Mab H19. Some strains were also reactive with Mab T65 in IFA while there was 'G' at base #326 and glycine at aa#109, but the other strains, which had 'A' at base #326 and glutamic acid at aa#109, did not react with Mab T65. By comparison of CVI988 to its point mutants CVI/rpp38(AG) and CVI/rpp38(AA) with 1 or 2 base(s) changes at bases #320 and /or #326 of pp38 gene for their reactivity with Mab H19 and T65, it was confirmed that the glutamine at aa#107 and glycine at aa#109 were critical to epi-topes H19 and T65 respectively. Immuno-reactions to MDV were compared in SPF chickens inoculated with cloned CVI988 and its mutant CVI/rpp38(AG). It was found that antibody responses to MDV in chickens inoculated with CVI/rpp38(AG) were delayed and significantly lower than that in chickens inoculated with the native CVI988. By differential comparison of antibody titers to different antigens, a third epitope specific to CVI988 and dependent on arginine at aa#107 was suggested to be responsible for the big difference in antibody responses induced by native CVI988 and its mutant.
%K Marek's disease virus
%K pp38 gene
%K point mutant
%K epitopes
%K antibody responses<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=55C7E24A9DDD1D85495CD4CD1156D777&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=CA4FD0336C81A37A&sid=0D0D661F0B316AD5&eid=C753EB8AC8F551B9&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=29