%0 Journal Article
%T Improved High-efficiency System for Rice Transformation Using Mature Embryo-derived Calli<br>一种改进的水稻成熟胚愈伤组织高效基因转化系统
%A Hui Chen
%A Yuan Zhao
%A Kang Chong
%A <br>陈惠
%A 赵原
%A 种康
%J 植物学报
%D 2008
%I 
%X Rice has been transformed via Agrobacterium transfection with use of mature embryo-derived calli, but the transformation efficiency has remained relatively low in general. In this study, we optimized different factors affecting transformation and established a highly efficient transformatiom system mediated by Agrobacterium using mature embryo-derived calli from two japonica cultivars (Zhonghua 10, 11). The transformation was performed with A. tumefaciens strain EHA105 harboring the plasmid pUN1301/OsRAA1, GUS used as the reporter gene and HPT as the selectable marker gene. First, we established a high-frequency regeneration system for rice, defined NBD2 (NB+2 mg·L-1 2,4-D) as callus induction medium, NBD0.5 as subculture medium, and RE1 (MS+1 mg·L-1 6-BA+0.25 mg·L-1 NAA+0.5 mg·L-1 KT+0.2 mg·L-1 ZT) and RE2 (MS+1 mg·L-1 6-BA+0.5 mg·L-1 NAA+0.5 mg·L-1KT+0.2 mg·L-1 ZT) as regeneration medium. Second, factors such as the selection of explants, the pre-culture medium and the subculture times were shows to affect the efficiency of T-DNA delivery. The transformation efficiency using the optimized method was increased to 70%, which was measured either by counting the number of hygromycin-resistant calli or the number of transgenic plants.
%K <br>农杆菌介导的遗传转化
%K 成熟胚诱导的愈伤组织
%K 水稻
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=4C4E92DCAD187381E039A79902014BD6&yid=67289AFF6305E306&vid=C5154311167311FE&iid=38B194292C032A66&sid=3224764AEAFCF8C2&eid=717CC18E05F2AFA0&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=24