%0 Journal Article
%T Molecular replacement studies on crystal structure of allophycocyanin from red algae Porphyra yezoensis
%A LIU Jinyu
%A JIANG Tao
%A ZHANG Jiping
%A CHANG Wenrui
%A LIANG Dongcai
%A <br>刘锦玉
%A 江涛
%A 张季平
%A 常文瑞
%A 梁栋材
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Using the crystal structure of allophycocyanin from cyanobacteriumSpirulina platensis (APC-SP) as a search model, the crystal structure of allophycocyanin from red algaePorphyra yezoensis (APC-PY) has been studied by molecular replacement methods. The APC-PY crystals (Form 3) belong to the space group of R32, cell dimensions a =b = 10.53 nm,c = 18.94 nm, α= β = 90 °, γ= 120 °; there is one αβ monomer in each crystallographic asymmetric unit in the cell. The translation function search gave a unique peak with a correlation coefficient (Cc) of 67.0% and an R-factor of 36.1 % for reflection data from 1.0 to 0.4 nm. Using the results by molecular replacement, the initial model of APC-PY was built, and the coincidence of the chromophore in APC-PY initial model with its2F o- Fc OMIT map further confirms the results by molecular replacement.
%K allophycocyanin
%K red algae
%K molecular replacement method<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=25B46D69D2BBCF00&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=38B194292C032A66&sid=EBD6B792C9111B87&eid=8BB50A069C48D50B&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15