%0 Journal Article
%T Construction of cDNA Library from the Callus of Saussrea medusa Maxim<br>水母雪莲愈伤组织cDNA文库的构建
%A JIN Zhi-Ping
%A ZHAO De-Xiu
%A QIAO Chuan-Ling
%A FU Chun-Xiang
%A <br>金治平
%A 赵德修
%A 乔传令
%A 付春祥
%J 植物学报
%D 2004
%I 
%X Total RNA of 1~15 d red line callus of S.medusa was extracted by TRIZOL Reagent. cDNA library was constructed with SMART cDNA Library Construction Kit. The primary library has a high titer from 1.5×106 to 4×106, in which 98% clones are recombinant and the insert cDNAs are from 0.5 kb to 3 kb. The amplified library has a titer of 1011. The positive signals of CHS, DFR and SmP gene were detected by PCR in the amplified library, though SmP gene was expressed at low abundance . This high quality cDNA library provides a useful tool for the study of the molecular mechanisms of the secondary metabolism of the flavonoids of S.medusa.
%K <br>水母雪莲
%K 愈伤组织
%K cDNA文库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=9D8E664895EAB0F7FA8A60E7717E44CF&yid=D0E58B75BFD8E51C&vid=659D3B06EBF534A7&iid=CA4FD0336C81A37A&sid=1D0FA33DA02ABACD&eid=8BD23BD67BF01A5C&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=7