%0 Journal Article
%T Screening of proteins that interact with human thrombopoietin receptor c-Mpl using yeast two-hybrid system
%A ZHAO Xinyan
%A FENG Libing
%A ZHOU Weiguo
%A DAI Weilie
%A LI Changben
%A ZHAO Shouyuan
%A <br>赵新燕
%A 冯丽冰
%A 周伟国
%A 戴卫列
%A 李昌本
%A 赵寿元
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Thrombopoietin (TPO) is the major cytokine involved in platelet production and exerts its effects via the receptor c-Mpl. The yeast two-hybrid system has been used to screen the proteins interacting with c-Mpl. First, the cDNA fragment of c-Mpl intracellular domain was cloned into two-hybrid vector pAS2, and the resulting plasmid is designated as pASMM. Then a human placenta cDNA library was screened using the pASMM as a target plasmid. Seven positive clones were isolated from 150 000 independent transformants. Sequence analysis of one of the positive clones demonstrates that a part of coding sequence of vimentin from 611 bp to 3' end and flanking non-translation region was obtained. Therefore, there is an interaction between vimentin and TPO receptor. The results suggest that cytoskeletal protein may play an important role in TPO signal transduction pathway.
%K thrombopoietin receptor c-Mpl
%K yeast two-hybrid system
%K vimentin
%K interaction<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=F30009069BA5DD61&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=38B194292C032A66&sid=1DF3F9D75A12D97B&eid=5B5B75F4854B8331&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=13