%0 Journal Article
%T Nuclear lamina in plant cells
%A WANG Jian
%A YANG Cheng
%A ZHAI Zhonghe
%A <br>汪健
%A 杨澄
%A 翟中和
%J 中国科学C辑(英文版)
%D 1996
%I Springer
%X By using selective extraction and diethylene glycol distearate (DGD) embedment and embedment-free electron microscopy, the nuclear lamina was demonstrated in carrot and Ginkgo male generative cells. Western blotting revealed that the nuclear lamina was composed of A-type and B-type lamins which contained at least 66-ku and 84-ku or 66-ku and 86-ku polypeptides, respectively. These lamin proteins were localized at the nudear periphery as shown by immunogold-labelling. In situ hybridization for light microscope and electron microscope showed that plant cells have the homologous sequences of animal lamin cDNA. The sorting site of lamin mRNA is mainly distributed in the cytoplasm near the nudear envelope. The data have verified that there indeed exists nudear lamina in plant cells.
%K plant cells
%K nnclear lamina
%K immunogold-labelling
%K in situ hybridization
%K mRNA sorting<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=6F86B9C6ED36F30C83A26314CDE07F82&yid=8A15F8B0AA0E5323&vid=7C3A4C1EE6A45749&iid=94C357A881DFC066&sid=78BF76CF5B7CB0F2&eid=D0E8F9CBDBE0070C&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=0