%0 Journal Article
%T Human pescadillo induces large-scale chromatin unfolding
%A ZHANG Hao
%A FANG Yan
%A HUANG Cuifen
%A YANG Xiao
%A YE Qinong
%A <br>ZHANG Hao
%A FANG Yan
%A HUANG Cuifen
%A YANG Xiao & YE Qinong Beijing Institute of Biotechnology
%A Beijing
%A China
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X The human pescadillo gene encodes a protein with a BRCT domain. Pescadillo plays an important role in DNA synthesis, cell proliferation and transformation. Since BRCT do- mains have been shown to induce chromatin large-scale unfolding, we tested the role of Pesca- dillo in regulation of large-scale chromatin unfolding. To this end, we isolated the coding region of Pescadillo from human mammary MCF10A cells. Compared with the reported sequence, the isolated Pescadillo contains in-frame deletion from amino acid 580 to 582. Targeting the Pesca- dillo to an amplified, lac operator-containing chromosome region in the mammalian genome re- sults in large-scale chromatin decondensation. This unfolding activity maps to the BRCT domain of Pescadillo. These data provide a new clue to understanding the vital role of Pescadillo.
%K Pescadillo
%K large-scale chromatin unfolding
%K replication<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=90D11DB539C9DD4383A8EC1960798D0B&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=38B194292C032A66&sid=B7BFA4B351E4C682&eid=44A4891E33BFF455&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15