%0 Journal Article
%T Assembly and structure of neurofllaments isolated from bovine spinal cord<br>Assembly and structure of neurofilaments isolated from bovine spinal cord
%A TONG Xiangjun CHEN Jianguo LIU Jie PANG Shijin ZHAI Zhonghe
%A <br>佟向军
%A 陈建国
%A 刘洁
%A 庞世瑾
%A 翟中和
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X Neurofilaments (NFs) are neuron-specific intermediate filaments. The NFs were isolated from bovine spinal cord by differential centrifugation. The NFs were detected with electron microscopy and scanning tunneling microscopy (STM) . Under STM, two kinds of sidearm of NFs were revealed: one was short, the other was long. They were arrayed along the 10-nm width core filaments one by one. The intervals between two adjacent long sidearms or two short sidearms were 20-22 nm, while those between two adjacent long and short sidearms were 10-ll nm. It was proposed that the rod domain of NF triplet proteins was 3/4-staggered. The assembly properties of NT triplet proteins were also studied. Immuno-colloidal-gold labeling assay showed that NF-M and NF-H are able to co-assemble into long filaments with NF-L. NF-M and NF-H can also co-constitute into winding filaments.
%K neurofilaments (NFs)
%K STM
%K sidearm
%K structure
%K assembly
%K immunogold<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=BAE9BEB09DBC987D32FD968E1CA522B4&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=FD7C952458BFB5D8&eid=1DF3F9D75A12D97B&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=0