%0 Journal Article
%T Isolation of development-related genes in somatic embryo radicle of carrot (Daucus carota L.) using modified cDNA RDA
%A DIAO Fengqiu
%A ZHANG Lei
%A HUANG Meijuan
%A WU Naihu
%A <br>刁丰秋
%A 张雷
%A 黄美娟
%A 吴乃虎
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Using modified cDNA RDA capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation, we have obtained four bands of specifically expressed cDNA in the carrot somatic embryo deregulated for 12 h, which were designated as NR-1, NR-2, NR-3 and NR-4, respectively. As revealed by homology analysis of their DNA sequences after cloning them into pBS, remarkable homology was demonstrated in NR-2, NR-3 and NR-4 with the genes coding for LEA (late embryogenesis abundant protein), Dna J and xyloglucan endo-trans-glycosylase in plants. On the other hand, NR-1 showing no homology with any known sequence may have come from unknown genes. Using 32P-labeled NR-1 as probe, hybridization with cDNA fragment population has shown that we have actually cloned a new gene fragment related to radicle development. As shown by further Southern hybridization, these genes may be present in carrot genome in the form of single or low copies.
%K carrot (Daucus carota L
%K )
%K cDNA RDA
%K radicle development
%K gene cloning<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=04EE834ECCE09767&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=CA4FD0336C81A37A&sid=F4B561950EE1D31A&eid=014B591DF029732F&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=20