%0 Journal Article
%T A novel recombinant adeno-associated virus vector packaging system with HSV-1 amplicon providing helper functions
%A SHU Yuelong
%A WU Xiaobing
%A YANG Tianzhong
%A GONG Huiyu
%A HOU Yunde
%A YAN Ziying
%A <br>舒跃龙
%A 吴小兵
%A 杨天忠
%A 贡惠宇
%A 侯云德
%A 颜子颖
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X A novel packaging system for producing recombinant adeno-associated virus (rAAV) vector was described. Instead of the conventional method for rAAV production by two-plasmid co-transfection followed by superinfec-tion with adenovirus 5, an HSV-1 amplicon system expressing AAV-2 rep and cap genes from their native promoters was used to provide complete helper functions for rAAV replicating and packaging. This HSV-1 amplicon stock consisted of two kinds of infectious HSV-1 virions, a replicating-defective HSV-1 amplicon pseudovirus harboring multi-copies of AAV-2 rep and cap gene and a temperature-sensitive HSV-1 mutant strain ts-KOS. High-liter rAAV was generated with this new packaging system. This packaging system gives a simple and scaleable process for rAAV production.
%K rAAV
%K HSV-1 amplicon
%K replication
%K packaging<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=B2F7195AD7B387A4056C98D7B2D3E7A3&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=94C357A881DFC066&sid=BC88D6B0750E09D1&eid=47F7649551A37CFC&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=1&reference_num=10