%0 Journal Article
%T Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes<br>Cloning,sequencing and function of sanA,a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes
%A JIA Junyong
%A LI Wenli
%A CHEN Wei
%A NIE Liping
%A TAN Huarong
%A <br>贾君永
%A 李文利
%A 陈蔚
%A 聂丽平
%A 谭华荣
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DMA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshli with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.
%K Streptomyces
%K nikkomycin biosynthesis gene
%K complementary cloning<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=4D85DFB08F9AAF77&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=CA4FD0336C81A37A&sid=340AC2BF8E7AB4FD&eid=16D8618C6164A3ED&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=2&reference_num=11