%0 Journal Article
%T Improvement of the Purification Method of Plant Glycolate Oxidase<br>植物乙醇酸氧化酶分离纯化方法的改进
%A Jinya Guo
%A Yuliang Dong
%A Jing Yang
%A Jie Xu
%A Hanping Yin
%A Qiulian Zeng
%A <br>郭晋雅
%A 董宇亮
%A 杨净
%A 徐杰
%A 尹汗萍
%A 曾秋莲
%J 植物学报
%D 2006
%I 
%X By reducing the length of diethylaminoethanol-cellulose and increasing the pH in elution solution,we developed an improved method for purifying glycolate oxidase with high isoelectric value (pI > 10.0) from green leaves of Spinacia oleracea, Brassica parachinensis, and Phaseolus vulgaris. Purified glycolate oxidase showed high specific activity (54.6-197.0 U.mg-1) and high pI (>10.0). We achieved 21.6-122.68-fold purification and 4.1%-71.5% yield from crude solution during this purification procedure. SDS-PAGE of the glycolate oxidases revealed a 40-kD band, which indicates that the subunit molecular weight of glycolate oxidases in the 3 different plants was identical.
%K <br>乙醇酸氧化酶
%K 纯化
%K 菠菜
%K 菜心
%K 豆角
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=5E6E3C259D3AD88BA9F95996F0D22C64&yid=37904DC365DD7266&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=7D2B339649A57040&eid=E008F9AD6D4B96EF&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0