%0 Journal Article
%T M16 Added with Taurine and EDTA Can Support the IVF and in vitro Development to Blastocyst of Kunming Strain Mouse<br>M16添加牛磺酸和EDTA支持昆明白小鼠体外受精并发育至囊胚 M16 Added with Taurine and EDTA Can Support the IVF and in vitro Development to Blastocyst of Kunming Strain Mouse
%A WANG Min-kang
%A LIU Ji-long
%A LI Guang-peng
%A LIAN Li
%A JIANG Yi-ping
%A ZHANG Tian
%A CHEN Da-yuan
%A <br>王敏康
%A 刘冀珑
%A 李光鹏
%A 廉莉
%A 江一平
%A 张田
%A 陈大元WANG Min-kang
%A LIU Ji-long
%A LI Guang-peng
%A LIAN Li
%A JIANG Yi-ping
%A ZHANG Tian
%A CHEN Da-yuan
%J 遗传
%D 2000
%I 
%X The results of our early experiments show that the 2 cell block can be overcome by culturing zygote in modified M16 ,modified CZB and TE medium.Our research shows that the taurine and EDTA play key role in overcoming 2 cell block in Kunming mouse.The results show that the addition of taurine and EDTA to M16 medium can support the IVF and develoment to blastocyst in vitro in Kunming strain mice.This is the first report that M16 medium added with taurine and EDTA can be used in both IVF and culture medium to overcome the 2 cell block of embryo development in Kunming strain mouse.
%K Kunming strain mouse
%K IVF
%K 2
%K cell block
%K taurine
%K EDTA<br>昆明鼠
%K 体外受精
%K 2－细胞阻滞
%K 牛磺酸
%K EDTA
%K 发育
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=8F737A04B5C4E852&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=DFEE4E8C33C95CEF&eid=119B6C0AA09DE6B9&journal_id=0253-9772&journal_name=遗传&referenced_num=14&reference_num=6