%0 Journal Article
%T Screening of microsatellite markers associated with cold tolerance of large yellow croaker (Pseudosciaena crocea R.)<br>大黄鱼耐低温性状相关微卫星标记的筛选
%A GAO Guo-Qiang
%A CHANG Yu-Mei
%A HAN Qi-Xia
%A CHI Bing-Jie
%A LI Ming-Yun
%A XUE Liang-Yi
%A LIANG Li-Qun Heilongjiang River Fisheries Research Institute
%A Chinese Academy of Fishery Sciences
%A Harbin
%A China
%A College of Fisheries
%A Life Sciences
%A Shanghai Ocean University
%A Shanghai
%A College of Life Sciences
%A Ningbo University
%A Ningbo
%A <br>高国强
%A 常玉梅
%A 韩启霞
%A 池炳杰
%A 李明云
%A 薛良义
%A 梁利群
%J 遗传
%D 2010
%I 
%X Cold tolerance is one of the major economic characters in fish. In order to discuss the cold tolerance of large yellow croaker (Pseudosciaena crocea R.), fifteen fluorescent dye-labeled microsatellite markers were applied to detect genetic differences between F1 offsprings of cold tolerance group and normal group of large yellow croaker by SSR-PCR. Each group contained 20 randomly and separately sampled individuals. As a result, marker LYC0002 five alleles (LYC0002104 bp, LYC0002106 bp, LYC0002108 bp, LYC0002110 bp, and LYC0002112 bp) were amplified with marker LYC0002 in both groups and 60% (12/20) of individuals had allele LYC0002112 bp in cold tolerance group exclusively, which indicated that this allele is probably sensitive to temperature and associated with gene for cold tolerance. In addition, four alleles (LYC0002106 bp, LYC0002108 bp, LYC0002110 bp, and LYC0002112 bp) were sequenced individually. Sequence alignments showed that LYC0002112 bp allele contains 10 (CA) repeats, the remaining three alleles lacked one (CA) one by one, corresponding to the stepwise mutation model (SMM) of microsatellite.
%K large yellow croaker (Pseudosciaena crocea R
%K )
%K cold tolerance
%K microsatellite
%K fluorescent dye<br>大黄鱼
%K 耐低温
%K 微卫星
%K 荧光染料
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=6F234629941AE76CAD69631B93864014&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=38B194292C032A66&sid=FBA00558C57D9C11&eid=82722E2B785EBF0D&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=28