%0 Journal Article
%T The Establishment of Method for Identifying SNP Genotype by CRS-PCR<br>应用创造酶切位点法检测单碱基突变 The Establishment of Method for Identifying SNP Genotype by CRS-PCR
%A Chun-Jiang Zhao
%A Ning Li
%A Xue-Mei Deng
%A <br>赵春江
%A 李宁
%A 邓学梅
%J 遗传
%D 2003
%I 
%X Created Restriction Site PCR (CRS-PCR) is a simple and efficient method to identify SNP genotypes. One or more mismatch bases are used in a primer to create a restriction site by combining SNP site after PCR. The CRS-PCR products can be genotyped with a way the same as PCR-RFLP. In the study, Extracellular fatty acid binding protein (EX-FABP) gene was served as an example for establishing the CRS-PCR method. Strategy of CRS-PCR was also discussed.
%K CRS-PCR
%K EX-FABP gene
%K genotyping of SNP<br>酶切位点
%K 单碱基
%K 突变
%K PCR-RFLP
%K DNA序列
%K 多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=78325264C242B3B4&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=38B194292C032A66&sid=170CE8B011EA4FD9&eid=C6EC7357BCACD3A4&journal_id=0253-9772&journal_name=遗传&referenced_num=24&reference_num=5