%0 Journal Article
%T Strategies for cloning of novel genes based on EST<br>基于EST的新基因克隆策略
%A LIU Yuan
%A CAI Jia-Bin
%A JIANG Guo-Song
%A TONG Qiang-Song
%A <br>刘媛
%A 蔡嘉斌
%A 蒋国松
%A 童强松
%J 遗传
%D 2008
%I 
%X Expressed sequence tags (EST) are short, randomly selected single-pass nucleotide sequence reads derived from cDNA libraries and represent a small part of a gene. Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes. Recently, because of the fast distension of EST databases, application of EST in gene mapping and cloning leads to revolutionary change in the strategies for cloning of novel genes. Despite of some insufficiencies, it has been proved that EST could promote the discovery and research of novel genes. In this article, an introduction about EST, especially EST-based strategies for cloning of novel genes will be given in details.
%K 表达序列标签
%K 基因克隆
%K 基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=4B5E9C357F1FB3FF2FDF2220A6478030&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=38B194292C032A66&sid=4290346F7268639E&eid=30897FA31CA3354D&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=37