%0 Journal Article
%T Factors that Influence Direct Sequencing of PCR Products<br>PCR产物直接测序技术中影响因素的研究
%A XU Zu-yuan
%A BAO Qi-yu
%A NIU Yu-xin Beijing Genomics Institute / Human Genome Center
%A <br>徐祖元
%A 包其郁
%A 牛宇欣
%J 遗传
%D 2002
%I 
%X Factors influenced direct sequencing of PCR (polymerase chain reaction) products were investigated in this paper. It showed that the specialization of PCR products played a key role in their sequencing reactions and only which could be sequenced directly. It also showed that the PCR reaction residues (including dNTP, primers, and metal ion) affected badly on the sequencing quality, so the purification of PCR products was necessary before sequencing. In addition,the optimum templates amount in sequencing reaction rose with the increasing of their DNA size in a certain range.
%K polymerase chain reaction(PCR)
%K direct sequencing of PCR product
%K ABI 377 -DNA sequencer
%K Q20<br>PCR产物
%K 直接测序技术
%K 影响因素
%K 聚合酶链反应
%K DNA自动测序仪
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=B4ABEA2CBA87A726&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=E5D85F291CED2DA6&eid=64808317C39DF331&journal_id=0253-9772&journal_name=遗传&referenced_num=7&reference_num=8