%0 Journal Article
%T Construction and Application of an in Silico Elongation System of Nucleic Acid Sequence<br>基于PC/Linux的核酸序列电子延伸系统的构建及其应用 Construction and Application of an in Silico Elongation System of Nucleic Acid Sequence
%A ZHANG Cheng-gang
%A SUN Huan-dong
%A OUYANG Shu-guang
%A WU Song-feng
%A HE Fu-chu
%A <br>张成岗
%A 孙焕东
%A 欧阳曙光
%A 吴松峰
%A 贺福初ZHANG Cheng-gang
%A SUN Huan-dong
%A OUYANG Shu-guang
%A WU Song-feng
%A HE Fu-chu
%J 遗传
%D 2002
%I 
%X Normally it is difficult to obtain full-length cDNA sequence of novel genes.More and more expressed sequence tags(ESTs) have been obtained since the start-up of human genome project.Powerful system is badly needed for data mining on these EST sequences.Based on a personal computer coupled with Linux operating system and EST database,the Blast software and Phrap software were used to construct a platform for in silico elongation of ESTs in our lab.The performance was tested using 11386 EST sequences and 511 partial-length cDNA sequences.Results demonstrated that 8373 EST and 389 cDNA sequence were elongated using this system.Thus the platform seems to be a fast way for full-length cDNA sequence cloning of new genes.
%K bioinformatics
%K expressed sequence tag
%K cDNA
%K in Silico sequence elongation
%K personal computer
%K Linux operation system<br>生物信息学
%K 表达序列标签
%K cDNA
%K 电子序列延伸
%K 微机
%K 核酸序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=DC7C42CC78A94996&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=771152D1ADC1C0EB&eid=318E4CC20AED4940&journal_id=0253-9772&journal_name=遗传&referenced_num=5&reference_num=13