%0 Journal Article
%T Cloning and optimizing expression of a periplasmic solute-binding gene gsiB from Escherichia coli<br>大肠杆菌细胞周质底物结合蛋白gsiB基因的克隆及其表达条件的优化
%A 王中山
%A 向泉桔
%A 王海燕
%A 张义正
%J 遗传
%D 2010
%I 
%X Cloning and expression of gsiB was carried out for studying protein structure and function of glutathione transport system. The coding sequence of Escherichia coli gsiB that encodes the periplasmic solute-binding protein of a glutathione transporter was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring GFP reporter gene through the method Sequence and Ligation–Independent Cloning (SLIC). The resulting recombinant plasmid pWaldo-GFP-GsiB was transformed into different E. coli strains and the expression conditions were optimized. It was found that E. coli BL21(DE3) was the best strain for gsiB gene expression among the four strains tested. Induction at lower incubation temperature of 18°C and 0.1 mmol/L of IPTG led to higher expression of gsiB in E. coli BL21(DE3). Western blotting analysis also showed the expression of gsiB and the molecular weight of expressed protein as expected.
%K 大肠杆菌
%K 谷胱甘肽转运系统
%K 细胞周质底物结合蛋白
%K gsiB基因
%K 基因表达
%K 蛋白质印迹
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=A238FE7D0E719DC60C13A9103582D4D5&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=94C357A881DFC066&sid=42D7028D961473F8&eid=B99A53AADE50D922&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=19