%0 Journal Article
%T Advances in Plant ProteomicsⅠ. Key Techniques of Proteome<br>植物蛋白质组学研究进展Ⅰ. 蛋白质组关键技术
%A RUAN Song-Lin
%A MA Hua-Sheng
%A WANG Shi-Heng
%A XIN Ya
%A QIAN Li-Hua TONG Jian-Xing
%A WANG Jie
%A <br>阮松林
%A 马华升
%A 王世恒
%A 忻 雅
%A 钱丽华
%A 童建新
%A 赵杭苹
%A 王 杰
%J 遗传
%D 2006
%I 
%X With the completion of genome sequences of the model plants, such as rice (Oryza sativa L.) and Arabidopsis thaliana, it has come into plant functional genomics era, which becomes a hard base of the appearance and development of plant proteomics. This review focuses on the background of proteomics, concept of proteomics and key techniques of proteomics. The key techniques of proteomics include separation, such as 2-DE (Two-Dimensional Electrophoresis), RP-HPLC (Reverse Phase High Performance Liquid Chromatography) and SELDI (Surface Enhanced Laser Desorption/ lonization) protein chip, mass spectrometry, such as MALDI-TOF-MS (Matrix Assisted Laser Desorption/lonization-Time Of Flight-Mass Spectrometry) and ESI-MS/MS (Electrospray lonization Mass Spectrometry/ Mass Spectrometry), databases related to proteomics, quantitative proteome, TAP (Tandem Affinity Purification) and yeast two-hybrid system. Challenges and prospects of proteomic techniques are discussed.
%K proteome
%K two-dimensional electrophoresis
%K mass spectrometry
%K yeast two-hybrid system
%K databases related to proteome<br>蛋白质组
%K 双向电泳
%K 生物质谱技术
%K 酵母双杂交系统
%K 蛋白质组相关数据库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=4CB1F376FC63F6E2&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=C62A2B4ABB9EF7A3&eid=7AC3CCCDC6EFABE9&journal_id=0253-9772&journal_name=遗传&referenced_num=8&reference_num=50