%0 Journal Article
%T In silico identification, molecular cloning and verification of a novel pig gene homologous to human BCL10 of in-nate immunity and its preliminary expression profiles in pigs<br>人天然免疫基因BCL10的猪同源物的识别、克隆与初步表达分析
%A HE Jian-Feng
%A ZHANG Yan-Ming
%A DUAN Hui-Juan
%A CAO Jin-Suo
%A ZHANG De-Li
%A <br>何建锋
%A 张彦明
%A 段会娟
%A 曹金锁
%A 张德礼
%J 遗传
%D 2008
%I 
%X We identified and characterized a novel swine gene Bcl10 with GenBank (Accession No. EU088132) which was homologous to human BCL10 (B-cell CLL/lymphoma 10) gene. The full-length cDNA of 925 bp for swine BCL10 was in silico cloned, and then its ORF of 702 bp coding 233 amino acid residues was verified by RT-PCR and DNA sequencing. NCBI BLAST assay indicated that this cDNA contained three extrons with a length of 57, 289 and 356 bp respectively, and it was located on the chromosome 4 of pig genome. Using semi-quantitative PCR, preliminary expression profiles of swine BCL10 were verified in different swine tissues. The expression of swine BCL10 was verified by GFP marker and RT-PCR assay. We found that, BCL10 expressed in high level in swine spleen, and with a modest level in thymus, brain and lymph node; low level mRNA was expressed in liver and not detectable level in kidney. The swine BCL10 gene was cloned to the GFP-containing eukaryotic expression vector pEGFP-C1 and transfected to PK-15 cell line by lipofectin. BCL10 was ex-pressed effectively in PK-15 cells. In summary, we cloned a novel swine BCL10 gene, and investigated its expression char-acters. This will be the fundament of the future research on its function.
%K BCL10基因
%K 克隆
%K 表达分析
%K 真核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=23F4A64B9AE53FB6DD648CB0D41424B4&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=B31275AF3241DB2D&sid=F4DCFB3CB96519BF&eid=9409F3EB075DCD5B&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=22