%0 Journal Article
%T Cloning and analyzing of rice blast resistance gene Pi-ta+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan<br>云南景洪直立型普通野生稻抗稻瘟病Pi-ta+等位基因的克隆与分析
%A GENG Xian-Sheng
%A YANG Ming-Zhi
%A HUANG Xing-Qi
%A CHENG Zai-Quan
%A FU Jian
%A SUN Tao
%A LI Jun
%A <br>耿显胜
%A 杨明挚
%A 黄兴奇
%A 程在全
%A 付坚
%A 孙涛
%A 李俊
%J 遗传
%D 2008
%I 
%X A 4,672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid sequence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics. Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.
%K 景洪直立型普通野生稻
%K Pi-ta
%K 等位基因
%K 基因克隆
%K DNA多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=E2C348EEA2FD6DCE98A12A49F19A486F&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=CA4FD0336C81A37A&sid=91C9056D8E8856E0&eid=DDD31293A7C7D057&journal_id=0253-9772&journal_name=遗传&referenced_num=4&reference_num=23