%0 Journal Article
%T Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli<br>天蚕抗菌肽Ａ与蜂毒素杂合肽基因的合成及在大肠杆菌中的克隆与表达 Cloning and Expression of a Gene Encoding Shortened Cecropin A-Melittin Hybrid in E.coli
%A ZHU Jia-ming
%A LIU Fei-peng
%A LI Yue-qing
%A ZHONG Zhen-yu
%A ZHANG Na
%A <br>朱嘉明
%A 刘飞鹏
%A 李月琴
%A 钟振宇
%A 张娜
%J 遗传
%D 2002
%I 
%X According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).
%K cecropin A
%K melittin
%K hybrid<br>天蚕抗菌肽A
%K 蜂毒素
%K 杂合肽
%K 基因克隆
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=21DFC7A9A6A70EB9&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=4AD960B5AD2D111A&eid=339D79302DF62549&journal_id=0253-9772&journal_name=遗传&referenced_num=10&reference_num=12