%0 Journal Article
%T Screening and polymorphism of Y chromosome microsatellite markers in swamp buffalo
沼泽型水牛Y染微卫星标记的筛选与多态性检测
%A ZHANG Xiao-Ming
%A YUE Xiang-Peng
%A ZHANG Chun-Mei
%A LAN Xian-Yong
%A CHEN Hong
%A LEI Chu-Zhao Shaanxi Key Laboratory of Molecular Biology for Agriculture
%A College of Animal Science
%A Technology
%A Northwest A & F University
%A Yangling
%A China
%A
张晓明
%A 乐祥鹏
%A 张春梅
%A 蓝贤勇
%A 陈宏
%A 雷初朝
%J 遗传
%D 2010
%I
%X To understand the genetic diversity of buffalo Y chromosome, 14 microsatellite markers specific for cattle Y chromosomes were used to analyze 31 male buffaloes of three populations (i.e., HH, BN, and PR) originating from southeastern Yunnan Province, P. R. China. The aim of this study was to examine the practicability of cattle Y chromosome-specific microsatellite markers in studying genetic diversity of buffalo Y chromosome. Three markers (INRA008, UMN0103, and UMN0504) produced single allele, and three (UMN1113, UMN0304, and BC1.2) produced three alleles. But these markers were all monomorphic. Another three markers (UMN0920, UMN0307, and UMN3008) amplified irregular ladder-like bands. These markers were not suitable for investigating the swamp buffalo Y chromosome genetic diversity. The remaining five microsatellites (INRA124, INRA189, BM861, PBR1F1, and UMN2001) were polymorphic, which can be used to study the swamp buffalo Y chromosome genetic diversity. The mean number of alleles (Na), expected heterozygosity (He), genetic diversity (GD), polymorphism information content (PIC), and Shannon's information index (SI) across these five polymorphic loci in the buffalo population samples studied were 2.8000, 0.3998, 0.4144, 0.3245, and 0.5849, respectively, demonstrating a moderate degree of polymorphism in the Y chromosome of the buffalo population.
%K swamp buffalo
%K Y chromosome microsatellite
%K genetic diversity
水牛
%K Y染色体微卫星标记
%K 遗传多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=9E35BAA4F6DCBD1C2EE2B0DF27BC60A8&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=38B194292C032A66&sid=0C191C6ECF79047F&eid=002786F01A86D891&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=15