%0 Journal Article
%T Activation tagging and its application in plant functional genomics research<br>激发标签技术在植物功能基因组研究中的应用
%A LI Zhi-Miao
%A YANG Yue-Jian
%A WANG Rong-Qing
%A ZHOU Guo-Zhi
%A RUAN Mei-Ying
%A YE Qing-Jing
%A YAO Zhu-Ping
%A <br>李志邈
%A 杨悦俭
%A 王荣青
%A 周国治
%A 阮美颖
%A 叶青静
%A 姚祝平
%J 遗传
%D 2008
%I 
%X It is a practical strategy to screen for mutants in the research of plant functional genomics. Comparing with classical T-DNA knock-out mutagenesis technology by the loss-of-function mutation, the activation T-DNA tagging technique based on the gain-of-function mutation has its own particular advantages, mainly characterized by producing dominant mutants of genes with functional redundancy and easily cloning of the genes. First, the basic principle of activation tagging, and the progress of its application in the research on plant functional genomics was reviewed, especially in Arabidopsis and rice. The recent research progress in the mechanism of plant biotic and abiotic stress tolerance and of plant development unraveled by the method of activation tagging was then addressed. Finally, the limitation and prospects of this technique were discussed.
%K 激发标签
%K 功能获得突变
%K 植物功能基因组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=72456EF7EE2390047B9AFAA5E0AF94CD&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=38B194292C032A66&sid=E2B9962CCD971A0D&eid=334C61CAF4C8EF4E&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=35