%0 Journal Article
%T Detection of the Integration of Human FⅨ(hFⅨ)on Chromosomes of Transgenic Mice by Fluorescence in situ Hybridization<br>应用荧光原位杂交检测人凝血因子Ⅸ在转基因小鼠染色体上的整合 Detection of the Integration of Human FⅨ（hFⅨ）on Chromosomesof Transgenic Mice by Fluorescence in situ Hybridization
%A XIAO Yan ping
%A XI Ying
%A HUANG Wen ying
%A HUANG Ying
%A <br>肖艳萍
%A 奚鹰
%A 黄文英
%A 黄英XIAO Yan-ping
%A XI Ying
%A HUANG Wen-ying
%A HUANG Ying
%J 遗传
%D 2002
%I 
%X Fluorescence in situ hybridization (FISH) was used to detect the integration of hFIX on chromosomes of transgenic mice from F1 to F4 generation in two strains. For transgenic mice, 98%-100% of metaphases and 85%-94% of interphases showed hybridization signal. For negative control mice,100% of metaphases and 95%-96% of interphases showed no hybridization signal. The results demonstrated that FISH developed to detect the integration sites of hFIX was high efficient and specific. The integration sites of the transgenic mice analyzed were both single but different between the two strains. The integration chromosomes can be found in the transgenic mice from F1 to F4 generation and the integration sites were the same as each of the strains,which indicated that the transgene was stably integrated and transmitted to offspring.
%K 荧光原位杂交
%K 检测
%K 人凝血因子Ⅸ
%K 转基因小鼠
%K 染色体
%K 整合位点
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=3CAECAA8E8E8FCA8&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=E1D946F217E3B046&eid=FBCA02DBD05BD4EA&journal_id=0253-9772&journal_name=遗传&referenced_num=8&reference_num=13