%0 Journal Article
%T 利用基因芯片对一高保守基因进行肿瘤相关分析 Tumor Relevance Analysis of A Highly Conserved Gene by Using Gene Microarray Hybridization
%A 程超
%A 周宗祥
%A 徐明
%A 赵炜
%A 徐坚
%A 曾立
%A 黄燕
%A 吴奇涵
%A 戴建锋
%A 应康
%A 谢毅
%A 毛裕民CHENG Chao
%A ZHOU Zong-xiang
%A XU Ming
%A ZHAO Wei
%A XU Jian
%A ZENG Li
%A HUANG Yan
%A WU Qi-han
%A DAI Jian-feng
%A YING Kang
%A XIE Yi
%A MAO Yu-ming
%J 遗传
%D 2002
%I 
%X 本文对大规模人类cDNA 测序过程中获得的一条高保守基因进行了初步功能研究，生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酵母中都有很高的保守性，其他分析预测该基因可能具有肿瘤相关性。RT-PCR分析表明，该基因在成人和胎儿组织中广谱表达。利用基因芯片分析该基因在7例肝癌、5例胰腺癌、2例喉癌和2例肺癌中表达情况，结果证实了该基因的肿瘤相关性，并且提示该基因在不同肿瘤类型中可能处于不同的地位。 Abstract:Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human、mouse、fruit fly、thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases、5 pancreas cancer cases、2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors.
%K 基因芯片
%K 保守性
%K 反转录-PCR
%K 结构域
%K Key
%K words
%K gene
%K microarray
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=EBAE3714695B6CF956AF9C7CC7C13528&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=0DEB7A8A66C33AAD&eid=E1D946F217E3B046&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=0