%0 Journal Article
%T Cloning, characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92<br>嗜碱芽孢杆菌PB92碱性蛋白酶基因启动子的克隆及应用
%A CHEN Kun
%A LI Ming
%A CHENG Kun
%A DU Lian-Xiang
%A ZHANG Tong-Cun
%A LU Fu-Ping
%A <br>陈坤
%A 黎明
%A 成堃
%A 杜连祥
%A 张同存
%A 路福平
%J 遗传
%D 2008
%I 
%X Abstract: Promoter function fragment of alkaline protease gene (GenBank accession number: EU130686) was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. Sequenced and analyzed revealed that it contains several typical pro-moter characterized regions. Two reverse translation frames were located in -538~-370 bp and-275~-128 bp. Deletion analysis of the sequence demonstrated that 414 bp to 619 bp upstream of the TSS showed predominant promoter activity, and a 105 bp length sequence can serve as this function. Additionally, a representative Sec-type signal peptide structure was detected in PB92 AprE signal peptide. By cloning the PaprE and alkaline protease signal peptide gene into pBE2, an expres-sion vector pBEAC was constructed, and a plant sweet protein monellin gene was highly expressed in B. subtilis 1A751.
%K TAIL-PCR<br>嗜碱芽孢杆菌PB92
%K TAIL-PCR
%K 碱性蛋白酶启动子
%K 信号肽
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=54A8A57D42289DB841488FDF8E63A484&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=708DD6B15D2464E8&sid=F3699C8B183A53ED&eid=E36FA098A813D3FB&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=15