%0 Journal Article
%T Comparison between Two Methods of Establishing DH Population for Mapping lox-3 Gene in Rice (Oryza sativa L.)<br>水稻耐贮藏基因（lox-3）DH作图群体构建方法的比较研究 Comparison between Two Methods of Establishing DH Population for Mapping lox-3 Gene in Rice (Oryza sativa L.)
%A CAO Li-yong
%A LOU Xiang-yang
%A SI Hua-min
%A HE Li-bin
%A SUN Zong-xiu
%A <br>曹立勇
%A 楼向阳
%A 斯华敏
%A 何立斌
%A 孙宗修CAO Li-yong
%A LOU Xiang-yang
%A SI Hua-min
%A HE Li-bin
%A SUN Zong-xiu
%J 遗传
%D 2002
%I 
%X Crosses were produced by using a storable rice variety (Daw Dam) and 2070,Ming Hui 63,as well as Dulur/Xie Qing Zao B as parents,among these materials there are abundant polymorphisms of DNA molecular markers.Their F 1's were applied to generate DH lines by an improved method of direct induction of pollen plant.The method of the direct induction of pollen plant was used as check treatment to evaluate the improved method.The results showed that the improved method increased the re-differentiation percentage of pollen callus,the percentage of regeneration plantlets,and the percentage of green regeneration plantlets and produced more vigorous seedlings.The improved method was a competent method of establishing DH population for mapping lox-3 gene.It was also showed that the efficiency of the new method was different among genotypes and out of three experimental crosses Daw Dam/2070 and Daw Dam/Minghui 63 showed more efficient.More than 60 DH lines have been obtained in this experiment.
%K storable gene in rice
%K an improved method for direct induction of pollen plant
%K anther culture
%K establishment of DH populationn<br>水稻
%K 耐贮藏基因
%K 改良
%K 一步成苗法
%K 花药培养
%K 加倍单倍体群体
%K 构建方法
%K 比较研究
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=55062F2FEC64D1E1&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=BA79719BCA7341D5&eid=F1177A9DF1349B63&journal_id=0253-9772&journal_name=遗传&referenced_num=4&reference_num=16