%0 Journal Article
%T Strategies of targeted gene replacement in filamentous fungi<br>丝状真菌目标基因替换过程中的策略与方法
%A WANG Jiao-Yu
%A DU Xin-Fa
%A CHAI Rong-Yao
%A SUN Guo-Chang
%A LIN Fu-Cheng
%A <br>王教瑜
%A 杜新法
%A 柴荣耀
%A 孙国昌
%A 林福呈
%J 遗传
%D 2007
%I 
%X Targeted gene replacement (TGR) is an important technique for gene function analysis. With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established. In this paper, strategies involved in TGR in filamentous fungi were reviewed, including transformation systems, targeting vector construction, mutant selection and so on. Compared with TGR, RNAi and other techniques of gene function analysis were introduced and reviewed as well.
%K targeted gene replacement
%K filamentous fungi
%K gene function analysis<br>目标基因替换
%K 丝状真菌
%K 基因功能分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=C96E9DAAA8BC3AAB&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=DF92D298D3FF1E6E&sid=9124D83E61CF1CD0&eid=46CB56AABC2765FF&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=38