%0 Journal Article
%T Construction of Chang Liver cDNA Library and Immunoscreening of ADAMS Related Genes<br>常氏肝癌细胞cDNA文库的构建及ADAMs相关基因的免疫筛选与序列分析
%A LIN Jun-Tang~
%A LI Yu-Chang~
%A ZHANG Hui-Yong~
%A XU Cun-Shuan~
%A <br>林俊堂
%A 李玉昌
%A 张会勇
%A 徐存拴
%J 遗传
%D 2004
%I 
%X Total RNA was isolated from Chang Liver cell. The full-length cDNA was synthesized using LD PCR by RNA 5' end switching mechanism, oligo(dT) as primer. SfiI digestion sites were introduced at both 3' and 5' end of cDNA. The cDNA was ligated with gammaTriplEX2 vector and packaged. Twenty-two positive clones were obtained by immunoscreening to the library, they were sequenced and blast in NCBI, one of them was identified a novel gene. It was submitted to GenBank and obtained accession number AY078070. It may lay down a foundation for studying on functions of ADAMs related gene.
%K Chang Liver
%K cDNA Library
%K ADAMs antibody
%K immunoscreening
%K gene cloning
%K BLAST<br>常氏肝癌细胞
%K cDNA文库
%K ADAMs抗体
%K 免疫筛选
%K 基因克隆
%K 同源检索
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=0838A58BB9761915&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=8225A9F184D4F1CA&eid=6920A1020E13BE87&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=10