%0 Journal Article
%T Growing of human embryonic stem cells on feeders derived from themselves<br>一种新的人胚胎干细胞自身来源的滋养层支持其体外培养
%A AN Shi-Min
%A ZENG Qiao
%A TENG Xiang-Yun
%A LONG Zhi-Gao
%A LI Juan
%A PAN Qian
%A WU Ling-Qian
%A LIANG De-Sheng
%A XIA Kun
%A XIA Jia-Hui
%A ZHANG Zhuo-Hua
%A <br>安世民
%A 曾桥
%A 滕祥云
%A 龙志高
%A 李娟
%A 潘乾
%A 邬玲仟
%A 梁德生
%A 夏昆
%A 夏家辉
%A 张灼华
%J 遗传
%D 2008
%I 
%X Abstract: This study was carried out to determine whether mesenchymal stem cells (MSCs) derived from teratoma of hu-man embryonic stem cells (hESCs) function as feeder cells to support hESCs growth. Approximately 5×106 hESCs were injected into the hind limb muscle of each SCID-beige mouse to form teratoma. After 8 weeks, the MSCs were isolated from the teratoma and cultured in Mesencult medium. Purified MSCs were then used as the feeder cells for hESCs culture. High purity MSCs derived from teratoma were isolated. The cells were morphologically similar to bone marrow MSCs (bMSCs). The teratoma-derived MSCs were negative for CD34 and CD45 but positive for CD29, CD49b, CD105, CD73, and CD90, which resembled those expressed by bMSCs. After passaged on MSCs feeder cells more than 10 passages, hESCs maintained hESC characteristics in morphology. Reverse PCR showed the expression of Oct4 and Nanog. SSEA-1 was negative and SSEA-4, TRA-1-60, and TRA-1-81 were positive. Alkaline phosphatase staining showed positive re-sults.The karyotype remained normal. Moreover, the hECSs cultured on teratoma-derived MSCs formed teratoma in vivo and embryoid body in vitro confirmed their pluripotency. Accordingly, MSCs derived from hESCs by in vivo differentiation can be used as the feeder cells for hESCs culture.
%K 人胚胎干细胞
%K 间充质干细胞
%K 滋养层
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=C4FE1E4CCA97CAEB83CE6EA920F0EEA4&yid=67289AFF6305E306&vid=340AC2BF8E7AB4FD&iid=59906B3B2830C2C5&sid=E30A3BF9610C35C9&eid=ECF40C1D1FB7C43B&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=17