%0 Journal Article
%T Dlmo Gene Encodes a 32 kDa Protein<br>Dlmo基因编码一个32kDa的蛋白 Dlmo Gene Encodes a 32 kDa Protein
%A ZHU Tian hui
%A QIN Gang
%A ZHANG Li dong
%A LIU Zhi pei
%A ZHANG Jie
%A <br>朱天慧
%A 秦刚
%A 张立冬
%A 刘志霈
%A 张杰
%A Brigitte Royer-PokoraZHU Tian-hui
%A QIN Gang
%A ZHANG Li-dong
%A LIU Zhi-pei
%A ZHANG Jie
%A Brigitte Royer-Pokora
%J 遗传
%D 2001
%I 
%X In order to gain some insight into a possible function of Dlmo gene,we used the TNT coupled reticulocyte lysate systems as an in vitro translation system to detect the Drosoplila protein. We found a 32kDa protein product.To demonstrate that the DLMO protein has the same size in vivo as in vitro we studied nuclear and cytoplasmic extracts from 2-4h embryos in Western blots. The immuno serum recognizes a 32kDa protein in the cytoplasm that is not present in the nucleus.The products were immune precipitated with the polyclonal anti-LMO2 antibody raised against the human protein and seen a positive band of 32kDa.Using immuno histochemical analysis was seen a positive staining in the basal cytoplasm of blastoderm embryos at 4 hours. This result confirms that the DLMO is a cytoplasmprotein, not like human LMO protein.
%K LIM
%K Dlmo
%K 转录因子
%K 胞浆蛋白
%K 原癌基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=56D27FABAE27DDF6&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=659D3B06EBF534A7&eid=B91E8C6D6FE990DB&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=8