%0 Journal Article
%T Protein Splicing and Its Application in Protein Engineering<br>蛋白质剪接及其在蛋白质工程中的应用Protein Splicing and Its Application in Protein Engineering
%A HE Dong-Mei~
%A <br>赫冬梅
%A 钱凯先
%A 沈桂芳
%A He Dong-Mei
%A Qian Kai-Xian
%A Shen Gui-Fang
%J 遗传
%D 2004
%I 
%X Protein splicing, which is an intein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions. Intein is an intervening polypeptide which can catalytic self-cleavage from a pre-protein accompanied by the concomitant joining of the two flanking polypeptides (the extein) through a peptide bond. Protein splicing not only enriches genetic theory of posttranslational processing, but also has a wide application prospect.
%K protein splicing
%K intein
%K extein<br>蛋白质剪接
%K 蛋白质内含肽
%K 蛋白质外显肽
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=691A3DD668E6ED7A&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=D537C66B6404FE57&eid=10828928EB89AD8E&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=22