%0 Journal Article
%T Application of enzyme-labeled probe in testing of transgenic plant<br>酶标探针在转基因植物检测中的应用
%A TU Zhi-Ming
%A CHEN Lin
%A YANG Guang-Xiao
%A HE Guang-Yuan
%A <br>涂知明
%A 陈泠
%A 杨广笑
%A 何光源
%J 遗传
%D 2007
%I 
%X Used alkaline-phosphatase-labeled DNA as a probe to examine the expression of foreign UidA gene in trans-genic plants.Alkaline phosphatase coupled with polyethyleneimine(PEI) using P-benzoquine as the cross-linking reagent was covalently linked to single-stranded DNA via glutraldehyde.Such DNA-enzyme complexes were used as a probe for dot hybridization and Southern blot.After hybridization and incubation with a substrate solution,results can be observed directly in three to six hours and the results showed that it was a sensitive,specific,rapid,safe and economical probe.Dot hybridization analysis showed that the UidA gene was transformed into the target plants and southern blot showed that there were at least 5 copies of UidA gene in transgenic plants.
%K tissue-specific promoter
%K UidA gene
%K hybridization
%K enzyme-labeled probe<br>组织特异性启动子
%K UidA基因
%K 杂交
%K 酶标探针
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=CD679EA997575907AC02DFDDD33A8FB1&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=59906B3B2830C2C5&sid=2838795948C22802&eid=05D0CF7AA2D40B14&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=11