%0 Journal Article
%T Molecular cloning and functional analysis of Bovine mammary gland matrix attachment regions<br>牛乳腺核基质附着区的克隆及其功能研究
%A YANG Rui-Feng
%A SHU Jian-Hong
%A LI Zhi-Yan
%A LIU Jin-Long
%A ZHANG Yong
%A <br>杨瑞锋 舒建洪 李志艳 刘金龙
%A 张涌
%J 遗传
%D 2006
%I 
%X The bovine genomic DNA was extracted from bovine blood, then bovine mammary gland matrix attachment region (BMARs) was cloned using a pair of primers, which were designed based on the related sequences in GenBank through bio-software Primer5.0 and Vector7.0. Upon preliminary analysis with bio-software, BMARs was TA cloned into PMD-18 T vector. By means of adding Kpn2 I and Xho I to 5' upstream of sensitive and antisensitive primers respectively, expressing vector BE was constructed after BMAR was cloned into the downstream of the reporter gene in pEGFP-C1. Bovine ear fibroblast cells were transfected by expressing vector BE with Lipofectamine.Compared with control bovine ear fibroblast cells transfected with pEGFP-C1, the effect of cloned BMR was apparent in dispelling position effect and enhancing gene expression.
%K matrix attachment regions
%K clone
%K cell expression<br>核基质附着区
%K 克隆
%K 细胞表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=DDEA0C4C1EF7EA49&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=CA4FD0336C81A37A&sid=4AD960B5AD2D111A&eid=6209D9E8050195F5&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=8