%0 Journal Article
%T Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants<br>甜蛋白Brazzein基因在番茄果实中的特异表达
%A YIN Tao
%A LU Hong-Yu
%A ZHANG Shang-Long
%A LIU Jing-Mei
%A CHEN Da-Ming
%A <br>尹涛
%J 遗传
%D 2009
%I 
%X The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vec-tor harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.
%K 番茄
%K 甜蛋白基因Brazzein
%K 遗传转化
%K 果实特异
%K AGPLI启动子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=C93744B36CD9B85F11728C054CEDDB01&yid=DE12191FBD62783C&vid=4AD960B5AD2D111A&iid=B31275AF3241DB2D&sid=46FF101E7ECF9F15&eid=06DAE5E1DF7D0B6A&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=17